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Single-step genome engineering in the bee gut symbiont Snodgrassella alvi

Lariviere PJ, Zuberi Ashraf AHM, Leonard SP, Miller LG, Moran NA, Barrick JE
bioRxiv 2023.09.19.558440 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
209540pBTK1050BTK Type 1 homology arm for Golden Gate assembly
209541pBTK1051BTK Type 5 homology arm for Golden Gate assembly
209544pBTK1054BTK assembled plasmid for integrating a KanR+E2-Crimson cassette into the S. alvi genome at a neutral site
209546pBTK1056BTK Type 4 antibiotic resistance cassette for Golden Gate assembly
209549pBTK1059BTK Type 5 homology arm for Golden Gate assembly
209550pBTK1061BTK assembled plasmid for integrating a KanR+E2-Crimson cassette into the S. alvi genome in place of the clpA gene

Antibodies from Article