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Chemogenetic Minitool for Dissecting the Roles of Protein Phase Separation.

Chung CI, Yang J, Shu X
ACS Cent Sci. 2023 Jul 7;9(7):1466-1479. doi: 10.1021/acscentsci.3c00251. eCollection 2023 Jul 26. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
208744CEL-eGFP-HoTag3part A of SparkDrop
208745ZIF-eGFP-HoTag6part B of SparkDrop
208746CEL-IFP2-SOScatcatalytic domain of SOS2 fused with near ir fluorescent protein and CEL tag
208747ZIF-RFP-CAAXmembrane anchored ZIF tag with red fluorescent protein
208748CEL-Frb-EGFP-HOTag3part A of SparkDrop-Frb
208749ZIF-NLS-EGFP(Y66F)-HOTag6part B of nls-SparkDrop with non-fluorescent eGFP
208751CEL-EGFP-YAPpart A of YAP/SPARK-ON
217611ZIF-NLS-EGFP*-HOTag6-T2A-CEL-EGFP-YAPLentiviral vector for constitutive expression of YAP/SPARK-ON (SFFV promoter)

Antibodies from Article