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Improved green and red GRAB sensors for monitoring dopaminergic activity in vivo.

Zhuo Y, Luo B, Yi X, Dong H, Miao X, Wan J, Williams JT, Campbell MG, Cai R, Qian T, Li F, Weber SJ, Wang L, Li B, Wei Y, Li G, Wang H, Zheng Y, Zhao Y, Wolf ME, Zhu Y, Watabe-Uchida M, Li Y
Nat Methods. 2023 Nov 30. doi: 10.1038/s41592-023-02100-w. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
208692pDisplay-GRAB-gDA3m-IRES-mCherry-CAAXExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m and a membrane-localized mcherry in mammalian cells
208693pDisplay-GRAB-gDA3h-IRES-mCherry-CAAXExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3h and a membrane-localized mcherry in mammalian cells
208694pDisplay-GRAB-rDA2m-IRES-EGFP-CAAXExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2m and a membrane-localized EGFP in mammalian cells
208695pDisplay-GRAB-rDA2h-IRES-EGFP-CAAXExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2h and a membrane-localized EGFP in mammalian cells
208696pDisplay-GRAB-rDA3m-IRES-EGFP-CAAXExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m and a membrane-localized EGFP in mammalian cells
208697pDisplay-GRAB-rDA3h-IRES-EGFP-CAAXExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h and a membrane-localized EGFP in mammalian cells
208698pAAV-hSyn-GRAB-gDA3mExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons
208699pAAV-hSyn-GRAB-gDA3hExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3h in neurons
208700pAAV-hSyn-GRAB-rDA2mExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2m in neurons
208701pAAV-hSyn-GRAB-rDA2hExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2h in neurons
208702pAAV-hSyn-GRAB-rDA3mExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m in neurons
208703pAAV-hSyn-GRAB-rDA3hExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h in neurons
208704pAAV-hSyn-GRAB-gDA3mutExpresses the genetically-encoded fluorescent dopamine (DA) control sensor GRAB_gDA3mut in neurons
208705pAAV-hSyn-GRAB-rDA2mutExpresses the genetically-encoded fluorescent dopamine (DA) control sensor GRAB_rDA2mut in neurons
208706pAAV-hSyn-GRAB-rDA3mutExpresses the genetically-encoded fluorescent dopamine (DA) control sensor GRAB_rDA3mut in neurons
208707pAAV-hSyn-DIO-GRAB-gDA3mExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons in a cre-dependent manner
208708pAAV-hSyn-DIO-GRAB-rDA3mExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m in neurons in a cre-dependent manner

Antibodies from Article