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A tool kit of highly selective and sensitive genetically encoded neuropeptide sensors.

Wang H, Qian T, Zhao Y, Zhuo Y, Wu C, Osakada T, Chen P, Chen Z, Ren H, Yan Y, Geng L, Fu S, Mei L, Li G, Wu L, Jiang Y, Qian W, Zhang L, Peng W, Xu M, Hu J, Jiang M, Chen L, Tang C, Zhu Y, Lin D, Zhou JN, Li Y
Science. 2023 Nov 17;382(6672):eabq8173. doi: 10.1126/science.abq8173. Epub 2023 Nov 17. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
208654pDisplay-GRAB_CRF0.5Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in mammalian cells
208655pDisplay-GRAB_CRF1.0Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in mammalian cells
208656pDisplay-GRAB_CRFmutExpresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut in mammalian cells
208657pAAV-hSyn-GRAB_CRF0.5Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in neurons
208658pAAV-hSyn-GRAB_CRF1.0Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in neurons
208659pAAV-hSyn-GRAB_CRFmutExpresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) control sensor GRAB_CRFmut in neurons
208660pAAV-hSyn-DIO-GRAB_CRF0.5Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in a cre-dependent manner
208661pAAV-hSyn-DIO-GRAB_CRF1.0Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in a cre-dependent manner
208662pAAV-EFS-DIO-GRAB_CRF1.0Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in a cre-dependent manner
208663pAAV-EFS-DIO-GRAB_CRFmutExpresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) control sensor GRAB_CRFmut in a cre-dependent manner
208664pDisplay-GRAB_SST1.0Expresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in mammalian cells
208665pAAV-hSyn-GRAB_SST1.0Expresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in neurons
208666pAAV-hSyn-DIO-GRAB_SST1.0Expresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in a cre-dependent manner
208667pDisplay-GRAB_UCN1.0Expresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in mammalian cells
208668pAAV-hSyn-DIO-GRAB_UCN1.0Expresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in neurons
208669pAAV-hSyn-GRAB_UCN1.0Expresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in a cre-dependent manner
208670pDisplay-GRAB_NTS1.0Expresses the genetically-encoded fluorescent neurotensin (NTS) sensor GRAB_NTS1.0 in mammalian cells
208671pAAV-EFS-DIO-GRAB_NTS1.0Expresses the genetically-encoded fluorescent neurotensin (NTS) sensor GRAB_NTS1.0 in a cre-dependent manner
208672pDisplay-GRAB_CCK1.0Expresses the genetically-encoded fluorescent cholecystokinin (CCK) sensor GRAB_CCK1.0 in mammalian cells
208673pAAV-hSyn-GRAB_CCK1.0Expresses the genetically-encoded fluorescent cholecystokinin (CCK) sensor GRAB_CCK1.0 in neurons
208674pAAV-hSyn-DIO-GRAB_CCK1.0Expresses the genetically-encoded fluorescent cholecystokinin (CCK) sensor GRAB_CCK1.0 in a cre-dependent manner
208675pDisplay-GRAB_NPY1.0Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in mammalian cells
208676pAAV-hSyn-GRAB_NPY1.0Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in neurons
208677pAAV-EF1_-DIO-GRAB_NPY1.0Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in a cre-dependent manner
208678pDisplay-GRAB_NPYmutExpresses the genetically-encoded fluorescent neuropeptide Y (NPY) control sensor GRAB_NPYmut in mammalian cells
208679pAAV-hSyn-GRAB_NPYmutExpresses the genetically-encoded fluorescent neuropeptide Y (NPY) control sensor GRAB_NPYmut in neurons
208680pAAV-hSyn-DIO-GRAB_VIP1.0Expresses the genetically-encoded fluorescent vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0 in a cre-dependent manner
208681pAAV-hSyn-GRAB_VIP1.0Expresses the genetically-encoded fluorescent vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0 in neurons
208682pDisplay-GRAB_VIP1.0Expresses the genetically-encoded fluorescent vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0 in mammalian cells
208683pDisplay-EGFP-CAAXExpresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in mammalian cells
208684pAAV-hSyn-EGFP-CAAXExpresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in neurons
208685pAAV-hSyn-DIO-EGFP-CAAXExpresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in a cre-dependent manner

Antibodies from Article