Efficient genome engineering in eukaryotes using Cas9 from Streptococcus thermophilus.
Xu K, Ren C, Liu Z, Zhang T, Zhang T, Li D, Wang L, Yan Q, Guo L, Shen J, Zhang Z
Cell Mol Life Sci. 2015 Jan;72(2):383-99. doi: 10.1007/s00018-014-1679-z. Epub 2014 Jul 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 208826 | pSSA-eGFP (pXK-221) | The SSA-based surrogate reporter for validating the activity of gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(SSA). |
| 208827 | pSSA-DsRed/eGFP (pXK-442) | The SSA-based surrogate reporter for validating the activity of different gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CMV-DsRed-CMV-eGFP*(SSA). |
| 208828 | pLL3.7-hSt3Cas9 (pXK-1721) | CRISPR/hSt3Cas9 expression vector for Animal gene editing, harboring the humanized St3Cas9 gene and the corresponding optimized SP1.gRNA. Key Construct: mU6- SP1.gRNA-CMV-hSt3Cas9. |