A single C-terminal residue controls SARS-CoV-2 spike trafficking and incorporation into VLPs.
Dey D, Qing E, He Y, Chen Y, Jennings B, Cohn W, Singh S, Gakhar L, Schnicker NJ, Pierce BG, Whitelegge JP, Doray B, Orban J, Gallagher T, Hasan SS
Nat Commun. 2023 Dec 15;14(1):8358. doi: 10.1038/s41467-023-44076-3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 208170 | SSH-pSUMO-β′-COPI-WD40-Tyr33Ala | Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40-Tyr33Ala mutant (residues 1-301) |
| 208172 | SSH-pcDNA3.1-α-COPI-WD40-Arg13Ala | Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 Arg13Ala mutant (residues 1-327) |
| 208173 | SSH-pcDNA3.1-α-COPI-WD40-Arg300Ala | Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg300Ala (residues 1-327) |
| 208174 | SSH-pcDNA3.1-α-COPI-WD40-Lys15Ala | Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Lys15Ala (residues 1-327) |
| 208341 | SSH-pSUMO-α-COPI-WD40 | Bacterial expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 |
| 218453 | JO-pH720-SARS-CoV-2-S-tail-WT | Bacterial expression plasmid for the cytosolic tail of SARS-CoV-2 spike with an N-terminal eXact tag |
| 218454 | JO-pH720-SARS-CoV-2-S-tail-Cys1254Ala-Thr1273Glu | Bacterial expression plasmid for the cytosolic tail of SARS-CoV-2 spike (with Cys1254Ala and Thr1273Glu substitutions) and an N-terminal eXact tag |
| 218457 | BD-pGEX5X3-BD-SARS-CoV-2-S-tail-Thr1273Asp | Bacterial expression plasmid for the SARS-CoV-2 cytosolic tail (Thr1273Asp substitution) with an N-terminal GST tag |
| 218460 | SSH-pcDNA3.1-SSH-SARS-CoV-2-S-WT | Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues) |
| 218461 | SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Glu | Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Glu substitution) |
| 218462 | SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Asp | Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Asp substitution) |
| 218463 | SSH-pcDNA3.1-SARS-CoV-2-S-Lys1269Ala-His1271Ala | Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Lys1269Ala and His1271Ala substitutions) |