BioE3 identifies specific substrates of ubiquitin E3 ligases.
Barroso-Gomila O, Merino-Cacho L, Muratore V, Perez C, Taibi V, Maspero E, Azkargorta M, Iloro I, Trulsson F, Vertegaal ACO, Mayor U, Elortza F, Polo S, Barrio R, Sutherland JD
Nat Commun. 2023 Nov 23;14(1):7656. doi: 10.1038/s41467-023-43326-8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 208042 | TRIPZ-bioGEF-SUMO1nc-PURO | Enables inducible expression of low-affinity Avitag-SUMO1nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin |
| 208043 | TRIPZ-bioGEF-SUMO2nc-PURO | Enables inducible expression of low-affinity Avitag-SUMO2nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin |
| 208044 | TRIPZ-bioGEF-UBnc-PURO | Enables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin |
| 208045 | TRIPZ-bioGEF-UBwt-PURO | Enables inducible expression of low-affinity Avitag-Ubwt; to perform bioE3; selection with puromycin |
| 208046 | Lenti-EFS-BirAopt-GSQ-RNF4wt-P2A-BLAST | Enables constitutive expression of N-terminal BirAopt-fused RNF4wt; to perform bioE3; selection with blasticidin |
| 208047 | Lenti-EFS-BirAopt-GSQ-RNF4ca-P2A-BLAST | Enables constitutive expression of N-terminal BirAopt-fused RNF4ca (catalytically inactive RING mutant); to perform bioE3; selection with blasticidin |
| 208048 | Lenti-EFS-BirAopt-GSQ-RBXN-P2A-BLAST | Enables constitutive expression of BirAopt alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; to perform bioE3; selection with blasticidin |