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Lactate biosensors for spectrally and spatially multiplexed fluorescence imaging.

Nasu Y, Aggarwal A, Le GNT, Vo CT, Kambe Y, Wang X, Beinlich FRM, Lee AB, Ram TR, Wang F, Gorzo KA, Kamijo Y, Boisvert M, Nishinami S, Kawamura G, Ozawa T, Toda H, Gordon GR, Ge S, Hirase H, Nedergaard M, Paquet ME, Drobizhev M, Podgorski K, Campbell RE
Nat Commun. 2023 Oct 27;14(1):6598. doi: 10.1038/s41467-023-42230-5. (Link opens in a new window) PubMed (Link opens in a new window) Article


Plasmids from this article have been deposited by multiple labs. See below for all materials from each lab:

  • Robert Campbell
  • Marie-Eve Paquet
  • Yuki Kambe

    • Plasmids from Article

    ID Plasmid Purpose
    207989pAAV-CaMKIIa-HA-eLACCO2.1-NGRBiosensor for extracellular L-lactate
    207990pAAV-CaMKIIa-HA-deLACCO1-NGRControl biosensor for extracellular L-lactate
    208017pBAD-eLACCO2.1Biosensor for extracellular L-lactate
    208018pBAD-deLACCO1Control biosensor for extracellular L-lactate
    208019pBAD-R-iLACCO1Biosensor for intracellular L-lactate
    208020pBAD-R-iLACCO1.1Biosensor for intracellular L-lactate
    208021pBAD-R-iLACCO1.2Biosensor for intracellular L-lactate
    208022pBAD-R-diLACCO1Control biosensor for intracellular L-lactate
    208023pcDNA-HA-eLACCO2.1-NGRBiosensor for extracellular L-lactate
    208024pcDNA-HA-deLACCO1-NGRControl biosensor for extracellular L-lactate
    208025pcDNA-R-iLACCO1Biosensor for intracellular L-lactate
    208026pcDNA-R-iLACCO1.1Biosensor for intracellular L-lactate
    208027pcDNA-R-iLACCO1.2Biosensor for intracellular L-lactate
    208028pcDNA-R-diLACCO1Control biosensor for intracellular L-lactate
    208096pAAV-hSyn-HA-eLACCO2.1-NGRBiosensor for extracellular L-lactate
    208097pAAV-hSyn-HA-deLACCO1-NGRControl biosensor for extracellular L-lactate
    208098pAAV-gfaABC1D-HA-eLACCO2.1-NGRBiosensor for extracellular L-lactate
    208099pAAV-gfaABC1D-HA-deLACCO1-NGRControl biosensor for extracellular L-lactate
    208100pAAV-hSyn-R-iLACCO1.1Biosensor for intracellular L-lactate
    208101pAAV-hSyn-R-iLACCO1.2Biosensor for intracellular L-lactate
    208102pAAV-hSyn-R-diLACCO1Control biosensor for intracellular L-lactate
    208103pAAV-gfaABC1D-R-iLACCO1.1Biosensor for intracellular L-lactate
    208104pAAV-gfaABC1D-R-iLACCO1.2Biosensor for intracellular L-lactate
    208105pAAV-gfaABC1D-R-diLACCO1Control biosensor for intracellular L-lactate

    Antibodies from Article