A SEVA-based, CRISPR-Cas3-assisted genome engineering approach for Pseudomonas with efficient vector curing.
Lammens E-M, Volke DC, Schroven K, Voet M, Kerremans A, Lavigne R, Hendrix H
Microbiol Spectr. 2023 Nov 17:e0270723. doi: 10.1128/spectrum.02707-23.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 207998 | pCas3-Amp | Expresses all necessary components of Type I-C CRISPR-Cas system |
| 207999 | pCas3-Km | Expresses all necessary components of Type I-C CRISPR-Cas system |
| 208000 | pCas3-Apr | Expresses all necessary components of Type I-C CRISPR-Cas system |
| 208001 | pCas3-Sm | Expresses all necessary components of Type I-C CRISPR-Cas system |
| 208002 | pSEVA13-GG | for cloning homology arms, with GFP as counter selection marker |
| 208013 | pSEVA23-GG | for cloning homology arms, with GFP as counter selection marker |
| 208014 | pSEVA43-GG | for cloning homology arms, with GFP as counter selection marker |
| 208015 | pSEVA63-GG | for cloning homology arms, with GFP as counter selection marker |
| 208016 | pSEVA83-GG | for cloning homology arms, with GFP as counter selection marker |