Dual-color DNA-PAINT single-particle tracking enables extended studies of membrane protein interactions.
Niederauer C, Nguyen C, Wang-Henderson M, Stein J, Strauss S, Cumberworth A, Stehr F, Jungmann R, Schwille P, Ganzinger KA
Nat Commun. 2023 Jul 19;14(1):4345. doi: 10.1038/s41467-023-40065-8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 200280 | pHR-SNAP-CD86tm-eGFP-FKBP(f36v) | Membrane protein fusion with extracellular SNAPtag and intracellular FKBP(f36v) dimerization domain and GFP |
| 200281 | pHR-eGFP-CD86tm-HALOtag-FKBP(f36v) | Membrane protein fusion with extracellular GFP and intracellular FKBP(f36v) dimerization domain and HALOtag |
| 200282 | pET30-10His-FKBP(f36v)-SNAP | For recombinant expression in E.coli (Rosetta) of a 10His-tag - FKBP(f36v) - SNAPtag fusion protein |
| 200283 | pLKO-puro SNAP-CD86-eGFP-FKBP(f36v) | Membrane protein fusion with extracellular SNAPtag and intracellular FKBP(f36v) dimerization domain and GFP reporter under Tetracycline-inducible promoter |