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High-throughput profiling of sequence recognition by tyrosine kinases and SH2 domains using bacterial peptide display.

Li A, Voleti R, Lee M, Gagoski D, Shah NH
Elife. 2023 Mar 16;12:e82345. doi: 10.7554/eLife.82345. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
214230pET28-His6-SUMO-cSrc-SH2-AviTagBacterial expression of SH2 domain of the cSrc kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

Antibodies from Article