High-throughput profiling of sequence recognition by tyrosine kinases and SH2 domains using bacterial peptide display.
Li A, Voleti R, Lee M, Gagoski D, Shah NH
Elife. 2023 Mar 16;12:e82345. doi: 10.7554/eLife.82345.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
214230 | pET28-His6-SUMO-cSrc-SH2-AviTag | Bacterial expression of SH2 domain of the cSrc kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections |