CRISPR single base-editing: in silico predictions to variant clonal cell lines.

Dickson KA, Field N, Blackman T, Ma Y, Xie T, Kurangil E, Idrees S, Rathnayake SNH, Mahbub RM, Faiz A, Marsh DJ
Hum Mol Genet. 2023 Jun 27:ddad105. doi: 10.1093/hmg/ddad105. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
229687	pCMV-eA3A-BE5-(NG)-PX458-EGFP	CRISPR CBE plasmid (C to T edits): eA3A-BE5-NG engineered to include the sgRNA cloning backbone from PX458 and EGFP. Includes BbsI sites for sgRNA cloning downstream of U6 promoter.