The SUMO-NIP45 pathway processes toxic DNA catenanes to prevent mitotic failure.

Hertz EPT, Vega IA, Kruse T, Wang Y, Hendriks IA, Bizard AH, Eugui-Anta A, Hay RT, Nielsen ML, Nilsson J, Hickson ID, Mailand N
Nat Struct Mol Biol. 2023 Jul 20. doi: 10.1038/s41594-023-01045-0. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
210262	EGFP-NIP45 wt	Inducible expression of N-terminally EGFP-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.
210263	EGFP-NIP45 delta-SLD2	Inducible expression of N-terminally EGFP-tagged NIP45 delta-SLD2. Use with Flp-In T-Rex system.
210264	EGFP-NIP45 delta-SLD1	Inducible expression of N-terminally EGFP-tagged NIP45 delta-SLD1. Use with Flp-In T-Rex system.
210265	EGFP-NIP45 D394R	Inducible expression of N-terminally EGFP-tagged NIP45 D394R resistant to siRNA. Use with Flp-In T-Rex system.
210266	mCherry-NIP45 wt	Inducible expression of N-terminally mCherry-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.
210267	mCherry-NIP45 D394R	Inducible expression of N-terminally mCherry-tagged NIP45 D394R resistant to siRNA. Use with Flp-In T-Rex system.
210268	EGFP-NIP45 delta-N1	Inducible expression of N-terminally EGFP-tagged NIP45 delta-N1 resistant to siRNA. Use with Flp-In T-Rex system.
210269	EGFP-NIP45 delta-N2	Inducible expression of N-terminally EGFP-tagged NIP45 delta-N2 resistant to siRNA. Use with Flp-In T-Rex system.
210270	pcDNA5-FRT-TO-EGFP-N	N-terminal EGFP-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.
210271	pcDNA5-FRT-TO-mCherry-N	N-terminal mCherry-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.