Generation of Amber Suppression Cell Lines Using CRISPR-Cas9.
Meineke B, Elsasser SJ
Methods Mol Biol. 2023;2676:169-180. doi: 10.1007/978-1-0716-3251-2_12.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 193309 | pX459_gRNA-AAVS1_hspCas9 | Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0) |
| 193310 | pX459_gRNA-ROSA26_hspCas9 | Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0) |
| 193311 | pX459_gRNA pAS single_hspCas9 | Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0) |
| 193312 | pX459_gRNA pAS dual_hspCas9 | Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0) |
| 193313 | pAS_sfGFP150K | pAS control plasmid for sfGFP expression |