The Ufd1 cofactor determines the linkage specificity of polyubiquitin chain engagement by the AAA+ ATPase Cdc48.
Williams C, Dong KC, Arkinson C, Martin A
Mol Cell. 2023 Mar 2;83(5):759-769.e7. doi: 10.1016/j.molcel.2023.01.016. Epub 2023 Feb 2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 224257 | pAM298 | MCM WT ubiquitin |
| 224258 | pAM299 | MCMUbHis |
| 226121 | pAM282 | Expression of cdc48 |
| 226122 | pAM284 | Expression of cdc48 E588Q |
| 226123 | pAM285 | Expression of cdc48 W561A/Y562A |
| 226125 | pAM288 | Expression of Npl4 W252A/R253E |
| 226126 | pAM289 | Expression of Npl4 D460K/Y461A |
| 226127 | pAM290 | Expression of His6-GST-3C-Npl4 |
| 226128 | paM291 | Expression of His6-GST-3C-Npl4 T255A/T571A |
| 226129 | pAM292 | Expression of His6-SUMO-Ub-mEos3.2-intein-CBD |
| 226130 | pAM294 | Expression of Ufd1-3C-His6 |
| 226131 | pAM295 | Expression of Ufd1R64C-3C-His6 |
| 226132 | pAM296 | Expression of Ufd1L55R-3C-His6 |
| 226134 | pAM301 | Expression of MC-Ub-SH3-3C-His6 |
| 226135 | pAM302 | Expression of MC-Ub-UbK48R-SH3-3C-His6 |
| 226136 | pAM303 | Expression of His6-GG-UbK48R |
| 226137 | pAM305 | Expression of His6-mEos3.2-UbK48R |
| 226138 | pAM358 | Expression of His6-SUMO-Ub-mEos3.2-mTurqoise2-intein-CBD |