Presynaptic targeting of botulinum neurotoxin type A requires a tripartite PSG-Syt1-SV2 plasma membrane nanocluster for synaptic vesicle entry.
Joensuu M, Syed P, Saber SH, Lanoue V, Wallis TP, Rae J, Blum A, Gormal RS, Small C, Sanders S, Jiang A, Mahrhold S, Krez N, Cousin MA, Cooper-White R, Cooper-White JJ, Collins BM, Parton RG, Balistreri G, Rummel A, Meunier FA
EMBO J. 2023 Jul 3;42(13):e112095. doi: 10.15252/embj.2022112095. Epub 2023 May 25.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 202553 | pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244) | Nontargeting control vector with TagBFP2 |
| 202554 | pLV-RnSyt1-sgRNA1-dCas9-KRAB-TagBFP2 (identifier AAAA-0245) | Synaptotagmin-1 sgRNA1 with TagBFP2 |
| 202555 | pLV-RnSyt1-sgRNA2-dCas9-KRAB-TagBFP2 (identifier AAAA-0246) | Synaptotagmin-1 sgRNA2 with TagBFP2 |
| 202556 | pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247) | Synaptotagmin-1 sgRNA3 with TagBFP2 |