The AAA+ ATPase Msp1 is a processive protein translocase with robust unfoldase activity.
Castanzo DT, LaFrance B, Martin A
Proc Natl Acad Sci U S A. 2020 Jun 30;117(26):14970-14977. doi: 10.1073/pnas.1920109117. Epub 2020 Jun 15.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 226888 | pAM244 | 6xHis-PSP-GS-Msp1(36-362) |
| 226889 | pAM245 | 6xHis-PSP-GS-PANN(75-150)-(5aaGS)-Msp1(36-362) |
| 226890 | pAM246 | 6xHis-PSP-GS-ccHex-(9aaSG)-Msp1(36-362) |
| 226891 | pAM247 | 6xHis-PSP-GS-Hcp1(1-162)-(6aaSG)-Msp1(36-362) |
| 226892 | pAM248 | 6xHis-PSP-GS-PANN(75-150)-(5aaGS)-Msp1(36-362)_W166A/Y167A |
| 226893 | pAM249 | Pex15(43-309)-PSP-GSS-FLAG-6xHis |
| 226894 | pAM250 | Pex15(1-253)-PSP-6xHis-FLAG |
| 226895 | pAM251 | MBP-Pex15(1-309)-PSP-GSS-FLAG-6xHis |
| 226896 | pAM252 | MBP-Pex15(1-327)-PSP-GSS-FLAG-6xHis |
| 226897 | pAM253 | mEOS3.2-PSP-GSS-FLAG-6xHis |
| 226898 | pAM254 | mEOS3.2-IDR*-(7aaSG)-PSP-GSS-FLAG-6xHis |
| 226899 | pAM255 | mEOS3.2-GS-MBP-10xN-Pex15(1-309)-PSP-GSS-FLAG-6xHis |
| 226900 | pAM256 | mEOS3.2-GS-SUMO-1(3-103)-Pex15(1-309)-PSP-GSS-FLAG-6xHis |
| 226901 | pAM257 | 6xHis-PSP-MBP-Pex3(40-441) |
| 226902 | pAM258 | 6xHis-PSP-MBP |
| 226903 | pAM259 | mEOS3.2-MBP-Pex15(1-253)-PSP-FLAG-6xHis |
| 226906 | pAM262 | 20aa^Pex15(1-253)-PSP-6xHis-FLAG |