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Coupled protein quality control during nonsense-mediated mRNA decay.

Inglis AJ, Guna A, Galvez-Merchan A, Pal A, Esantsi TK, Keys HR, Frenkel EM, Oania R, Weissman JS, Voorhees RM
J Cell Sci. 2023 May 15;136(10):jcs261216. doi: 10.1242/jcs.261216. Epub 2023 May 23. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
216220NMD2Nonsense mediated decay GFP P2A RFP reporter with two b-globin introns
216221NMD2_lentiNonsense mediated decay GFP P2A RFP reporter with two b-globin introns in lenti backbone
216222GPR_controlGFP P2A RFP control reporter
216223GPR_control_lentiGFP P2A RFP control reporter in a lenti backbone
216224inert_EJCGFP P2A RFP reporter with inert EJC immediately following stop codon

Antibodies from Article