Conditional lethality profiling reveals anticancer mechanisms of action and drug-nutrient interactions.
Flickinger KM, Wilson KM, Rossiter NJ, Hunger AL, Vishwasrao PV, Lee TD, Mellado Fritz CA, Richards RM, Hall MD, Cantor JR
Sci Adv. 2024 Oct 4;10(40):eadq3591. doi: 10.1126/sciadv.adq3591. Epub 2024 Oct 4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 217427 | pLJC2-TK2-3xFLAG | Lentiviral overexpression of human TK2 |
| 217428 | pLJC2-DHFR-3xFLAG | Lentiviral overexpression of human DHFR |
| 217429 | pLJC2-MTHFD1-3xFLAG | Lentiviral overexpression of human MTHFD1 |
| 217430 | pLentiCRISPR-v1-sgTYMS | Lentiviral vector expressing Cas9 and an sgRNA targeting human TYMS |
| 217431 | pLentiCRISPR-v1-sgTK2_4 | Lentiviral vector expressing Cas9 and an sgRNA targeting human TK2 |
| 217432 | pLentiCRISPR-v1-sgTK2_5 | Lentiviral vector expressing Cas9 and an sgRNA targeting human TK2 |
| 217433 | pLentiCRISPR-v1-sgABCC4_1 | Lentiviral vector expressing Cas9 and an sgRNA targeting human ABCC4 |
| 217434 | pLentiCRISPR-v1-sgABCC4_3 | Lentiviral vector expressing Cas9 and an sgRNA targeting human ABCC4 |
| 217435 | pLentiCRISPR-v1-sgSHMT2 | Lentiviral vector expressing Cas9 and an sgRNA targeting human SHMT2 |
| 217436 | pLentiCRISPR-v1-sgMTHFD1 | Lentiviral vector expressing Cas9 and an sgRNA targeting human MTHFD1 |