Translational fidelity screens in mammalian cells reveal eIF3 and eIF4G2 as regulators of start codon selectivity.
She R, Luo J, Weissman JS
Nucleic Acids Res. 2023 Jul 7;51(12):6355-6369. doi: 10.1093/nar/gkad329.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 222109 | pGFP-AUG-IRES-mCherry | Start codon reporter (WT AUG) |
| 222110 | pGFP-CUG-IRES-mCherry | Start codon reporter with mutated start codon |
| 222112 | pGFP-UUG-IRES-mCherry | Start codon reporter with mutated start codon |
| 222113 | pGFP-ACG-IRES-mCherry | Start codon reporter with mutated start codon |
| 222115 | pGFP-AGG-IRES-mCherry | Start codon reporter with mutated start codon |
| 222117 | pGFP-AUC-IRES-mCherry | Start codon reporter with mutated start codon |
| 222118 | pGFP-AUU-IRES-mCherry | Start codon reporter with mutated start codon |
| 222125 | pEIF3D-CtermDel-sgEIF3D | EIF3D variant overexpression vector with sgRNA targeting eIF3D |
| 222129 | pEIF3D-S528N-S529N-sgEIF3D | EIF3D variant overexpression vector with sgRNA targeting eIF3D |
| 222133 | pEIF3D-a5helixDel-sgEIF3D | EIF3D variant overexpression vector with sgRNA targeting eIF3D |
| 222135 | pEIF3D-a11helixDel-sgEIF3D | EIF3D variant overexpression vector with sgRNA targeting eIF3D |