Efficient CRISPR/Cas9-mediated gene editing in mammalian cells by the novel selectable traffic light reporters.
Lyu M, Sun Y, Yan N, Chen Q, Wang X, Wei Z, Zhang Z, Xu K
Int J Biol Macromol. 2023 Jul 15;243:124926. doi: 10.1016/j.ijbiomac.2023.124926. Epub 2023 May 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 208857 | pSSA-PMG (pXK-1649) | SSA-base selectable traffic light reporter (TLR) for helping to screen gene-editing positive cells. Key Construct: CMV-PuroR.L-mCherry-PuroR.R-GFP. |
| 208858 | pHDR-PMG (pXK-1650) | HDR-base selectable traffic light reporter (TLR) for helping to screen HDR-editing positive cells. Key Construct: iPuroR*-CMV-PuroR.L-mCherry-PuroR.R-GFP. |