A self-propagating, barcoded transposon system for the dynamic rewiring of genomic networks.
English MA, Alcantar MA, Collins JJ
Mol Syst Biol. 2023 Jun 12;19(6):e11398. doi: 10.15252/msb.202211398. Epub 2023 Mar 27.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 213902 | pDonor_Tn[sfGFP] | Mariner transposon expressing sfGFP with kan selection casette |
| 213903 | pDonor_Tn[GFP] | Mariner transposon expressing mNeonGreen with kan selection casette |
| 213904 | pDonor_Tn[RFP] | Mariner transposon expressing mCherry with cam selection casette |
| 213905 | pDonor_Tn-pOUT | Mariner transposon with outward-facing pJ23104 promoter |
| 213906 | pDonor_Tn[himar1C9] | Mariner transposon with ATc-inducible himar1C9 transposase |
| 213907 | pDonor_RB-TnV2 | Barcoded mariner transposon with kan selection casette, and modified terminal sequences - barcodes can be added via FseI-SbfI restriction sites |
| 213908 | pDonor_RB-TnV2_pJEx | Barcoded mariner transposon with crystal violet-inducible outward promoter - barcodes can be added via FseI-SbfI restriction sites |
| 213909 | pHelper_AraC/pBad-Tnpase (camR) | Arabinose-inducible himar1C9 transposase expression - cam resistance |
| 213910 | pHelper_TetR/pTet-Tnpase (camR) | ATc-inducible himar1C9 transposase expression - cam resistance |
| 213911 | pHelper_TetR/pTet-Tnpase (carbR) | ATc-inducible himar1C9 transposase expression - carb/amp resistance |