GreenGate 2.0: Backwards compatible addons for assembly of complex transcriptional units and their stacking with GreenGate.
Piepers M, Erbstein K, Reyes-Hernandez J, Song C, Tessi T, Petrasiunaite V, Faerber N, Distel K, Maizel A
PLoS One. 2023 Sep 8;18(9):e0290097. doi: 10.1371/journal.pone.0290097. eCollection 2023.
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Article
These materials are included in the GreenGate 2.0 Toolkit .
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 207885 | pR540 | template for 2 gRNA amplification |
| 207886 | pR562 | Creation of Level 0 entry clones |
| 207887 | pAM745 | Level 0 for A1 module (5' ACCT-3' TGAC) |
| 207888 | pAM749 | Level 0 for AD2 module (5' TGAC-3' TCCC) |
| 207889 | pAM750 | Level 0 for A3 module (5' TCCC-3' AACA) |
| 207890 | pAM751 | Level 0 for A4 module (5' TGAC-3' AACA) |
| 207891 | pAM752 | Level 0 for A5 module (5' ACCT-3' TCCC) |
| 207892 | pAM753 | Level 0 for B1 module (5' AACA-3' CCAT) |
| 207893 | pAM754 | Level 0 for B2 module (5' CCAT-3' GGCT) |
| 207894 | pAM755 | Level 0 for C1 module (5' GGCT-3' AGCC) |
| 207895 | pAM756 | Level 0 for C2 module (5' AGCC-3' TTCG) |
| 207896 | pAM757 | Level 0 for C3 module (5' TTCG-3' GCAG) |
| 207897 | pAM758 | Level 0 for C4 module (5' GCAG-3' TCAG) |
| 207898 | pAM759 | Level 0 for C5 module (5' AGCC-3' TCAG) |
| 207899 | pAM760 | Level 0 for C6 module (5' TTCG-3' TCAG) |
| 207900 | pAM761 | Level 0 for C7 module (5' GGCT-3' TTCG) |
| 207901 | pAM762 | Level 0 for C8 module (5' GGCT-3' GCAG) |
| 207902 | pAM763 | Level 0 for D1 module (5' TCAG-3' TGAC) |
| 207903 | pAM764 | Level 0 for D3 module (5' TCCC-3' CTGC) |
| 207904 | pAM765 | Level 0 for D4 module (5' TGAC-3' CTGC) |
| 207905 | pAM766 | Level 0 for D5 module (5' TCAG-3' TCCC) |
| 207906 | pAM767 | Level 0 for E1 module (5' CTGC-3' CCAT) |
| 207907 | pAM768 | Level 0 for E2 module (5' CCAT-3' ACTA) |
| 207908 | pKE014 | GG entry for zCas9i expression |
| 207909 | pCS017 | Level 0 for gRNA in C1 module (5' GGCT-3' AGCC) |
| 207910 | pKE010 | Level 0 for gRNA in C2 module (5' AGCC-3' TTCG) |
| 207911 | pCS018 | Level 0 for gRNA in C3 module (5' TTCG-3' GCAG) |
| 207912 | pKE011 | Level 0 for gRNA in C4 module (5' GCAG-3' TCAG) |
| 207913 | pCS019 | Level 0 for gRNA in D1 module (5' TCAG-3' TGAC) |
| 207914 | pKE012 | Level 0 for gRNA in AD2 module (5' TGAC-3' TCCC) |
| 207915 | pCS020 | Level 0 for gRNA in D3 module (5' TCCC-3' CTGC) |
| 207916 | pKE013 | Level 0 for gRNA in E1 module (5' CTGC-3' CCAT) |
| 207917 | pCS021 | Level 0 for gRNA in E2 module (5' CCAT-3' ACTA) |
| 207918 | pMP116 | GreenBraid destination vector pGBZ1 - PaqCI - Eco31I - spisPink- Eco31I - PaqCI |
| 207919 | pMP120 | GreenBraid reverse destination vector pGBZ1r - PaqCI - Eco31I - spisPink- Eco31I - PaqCI |
| 207920 | pMP117 | GreenBraid destination vector pGBZ2 - PaqCI - Eco31I - spisPink- Eco31I - PaqCI |
| 207921 | pMP121 | GreenBraid reverse destination vector pGBZ2r - PaqCI - Eco31I - spisPink- Eco31I - PaqCII |
| 207922 | pMP072 | GreenBraid joker destination vector pGBZ1j - PaqCI - dummy - PaqCI |
| 207923 | pMP073 | GreenBraid joker destination vector pGBZ2j - PaqCI - dummy - PaqCI |
| 207924 | pTT135 | Premade UBI10 driven expression of LhG4 (pUBQ10:GR-LhG4:tHSP18.2M) for braiding |
| 207925 | pMP107 | Premade plasma membrane marker, pUBQ10:PiP1;4:PmScarlet-I:tHSP18.2M, for braiding |
| 207926 | pMP104 | Premade plasma membrane marker, pUBQ10:PiP1;4:mVenus:tHSP18.2M, for braiding |
| 207927 | pMP105 | Premade plasma membrane marker, pUBQ10:PiP1;4:PmScarlet-I:tHSP18.2M, for braiding |
| 207928 | pMP108 | Premade plasma membrane marker, pUBQ10:PiP1;4:PmScarlet-I:tHSP18.2M:FastRed, for braiding |
| 207929 | pMP065 | Premade plasma membrane marker, pUBQ10:PiP1;4:mVenus:tHSP18.2M, for braiding |
| 207930 | pMP086 | Premade plasma membrane marker, pUBQ10:PiP1;4:mVenus:tHSP18.2M:FastRed, for braiding |
| 207931 | pKD013 | Premade plasma membrane marker, pUBQ10:PiP1;4:mTurquoise2:tHSP18.2M, for braiding |
| 207932 | pKD016 | Premade plasma membrane marker, pUBQ10:PiP1;4:mTurquoise2:tHSP18.2M:FastRed, for braiding |
| 207933 | pKD014 | Premade plasma membrane marker, pUBQ10:PiP1;4:mTurquoise2:tHSP18.2M, for braiding |
| 207934 | pKD015 | Premade nuclei membrane marker, pUBQ10:PiP1;4:mTurquoise2:tHSP18.2M:FastRed, for braiding |
| 207935 | pKD011 | Premade nuclei membrane marker, pUBQ10:H2B:mTurquoise2:tHSP18.2M, for braiding |
| 207936 | pKD005 | Premade nuclei membrane marker, pUBQ10:H2B:mTurquoise2:tHSP18.2M:FastRed, for braiding |
| 207937 | pKD012 | Premade nuclei membrane marker, pUBQ10:H2B:mTurquoise2:tHSP18.2M, for braiding |
| 207938 | pKD006 | Premade nuclei membrane marker, pUBQ10:H2B:mTurquoise2:tHSP18.2M:FastRed, for braiding |
| 207939 | pKD009 | Premade nuclei membrane marker, pUBQ10:H2B:mVenus:tHSP18.2M, for braiding |
| 207940 | pKD003 | Premade nuclei membrane marker, pUBQ10:H2B:mVenus:tHSP18.2M:FastRed, for braiding |
| 207941 | pKD010 | Premade nuclei membrane marker, pUBQ10:H2B:mVenus:tHSP18.2M, for braiding |
| 207942 | pKD004 | Premade nuclei membrane marker, pUBQ10:H2B:mVenus:tHSP18.2M:FastRed, for braiding |
| 207943 | pKD007 | Premade nuclei membrane marker, pUBQ10:H2B:PmScarlet-I:tHSP18.2M, for braiding |
| 207944 | pKD001 | Premade nuclei membrane marker, pUBQ10:H2B:PmScarlet-I:tHSP18.2M:FastRed, for braiding |
| 207945 | pKD008 | Premade nuclei membrane marker, pUBQ10:H2B:PmScarlet-I:tHSP18.2M, for braiding |
| 207946 | pKD002 | Premade nuclei membrane marker, pUBQ10:H2B:PmScarlet-I:tHSP18.2M:FastRed, for braiding |
| 207947 | pMP118 | GreenBraid destination vector pGBY1 - Eco31I - PaqCI -sfGFP- PaqCI - Eco31I |
| 207948 | pMP122 | GreenBraid reverse destination vector pGBY1r - Eco31I - PaqCI -sfGFP- PaqCI - Eco31I |
| 207949 | pMP119 | GreenBraid destination vector pGBY2 - Eco31I - PaqCI -sfGFP- PaqCI - Eco31I |
| 207950 | pMP123 | GreenBraid reverse destination vector pGBY2r - Eco31I - PaqCI -sfGFP- PaqCI - Eco31I |
| 207951 | pMP074 | GreenBraid joker destination vector pGBY1j - Eco31I - dummy - Eco31I |
| 207952 | pMP075 | GreenBraid joker destination vector pGBY2j - Eco31I - dummy - Eco31I |