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Permissive Conformations of a Transmembrane Helix Allow Intramembrane Proteolysis by gamma-Secretase.

Ortner M, Guschtschin-Schmidt N, Stelzer W, Muhle-Goll C, Langosch D
J Mol Biol. 2023 Aug 1;435(18):168218. doi: 10.1016/j.jmb.2023.168218. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
207801pYea5.1_Notch1_sfGFPYeast 2µ vector for expression of Suc2SP-Notch1_100-sfGFP-GAL4
207802pYea5.1_C99_sfGFPYeast 2µ vector for expression of Suc2SP-C99-sfGFP-GAL4
207803pRS405-FlagPEN2/PS1wtYeast integrative vector for expression of FlagPEN2 and Presenilin 1
207804pRS402-nicastrin/APH-1a-HAYeast integrative vector for expression of Nicastrin and APH-1a-HA
207864pYea5.1_Notch3_sfGFPYeast 2µ vector for expression of Suc2SP-Notch3_100-sfGFP-GAL4
207865pYea5.1_Notch1-L24_sfGFPYeast 2µ vector for expression of Suc2SP-Notch1-L24_100-sfGFP-GAL4

Antibodies from Article