Metabolic flux optimization of iterative pathways through orthogonal gene expression control: Application to the beta-oxidation reversal.
Lee SH, Hu Y, Chou A, Chen J, Gonzalez R
Metab Eng. 2024 Mar;82:262-273. doi: 10.1016/j.ymben.2024.02.007. Epub 2024 Feb 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 207672 | pCEntry | Entry vector for Trio vector system, with cumate-inducible promoter |
| 207673 | pPEntry | Entry vector for Trio vector system, with DAPG-inducible promoter |
| 207674 | pVEntry | Entry vector for Trio vector system, with vanillate-inducible promoter |
| 207675 | pACYC Destination | Trio “destination vectors” which harbor an operon for constitutive expression of the repressors for each inducible promoter (CymR, PhlF, and VanR for cumate, DAPG and vanillate, respectively). |