Intein-based thermoregulated meganucleases for containment of genetic material.

Foo GW, Leichthammer CD, Saita IM, Lukas ND, Batko IZ, Heinrichs DE, Edgell DR
Nucleic Acids Res. 2024 Feb 28;52(4):2066-2077. doi: 10.1093/nar/gkad1247. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
207883	pDual	Expresses thermosensitive variants of the homing endonucleases I-OnuI and I-GpeMI, each in a different open reading frame. Both were made thermosensitive by a fusion with the L212P VMA1 intein.
207884	pTarget-Dual	Carries the cognate target sites for the homing endonucleases I-OnuI and I-GpeMI.
207954	pEndo-I-OnuI-TSM	Expresses a fusion of the homing endonuclease I-OnuI with the thermosensitive L212P VMA1 intein. Endonuclease activity is restricted to lower temperatures.