Engineered bacteria titrate hydrogen sulfide and induce concentration-dependent effects on the host in a gut microphysiological system.
Hayes JA, Lunger AW, Sharma AS, Fernez MT, Carrier RL, Koppes AN, Koppes R, Woolston BM
Cell Rep. 2023 Dec 26;42(12):113481. doi: 10.1016/j.celrep.2023.113481. Epub 2023 Nov 18.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 207649 | pBbA5c-cdl | Overexpression of heterologous L-cysteine desulfidase in E. coli MG1655 |
| 207650 | pACYC-yhaM | Overexpression of native L-cysteine desulfidase in E. coli MG1655 |
| 207651 | pACYC-yhaM-RFP | Overexpression of native L-cysteine desulfidase and RFP in E. coli MG1655 |
| 207652 | pACYC-cdl | Overexpression of heterologous L-cysteine desulfidase in E. coli MG1655 |
| 207653 | pACYC-decR | Overexpression of L-cysteine desulfidase transcriptional regulator in E. coli MG1655 |
| 207654 | pMF2-yhaO | Overexpression of L-cysteine importer in E. coli MG1655 |