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Single-molecule imaging reveals the kinetics of non-homologous end-joining in living cells.

Mikhova M, Goff NJ, Janovic T, Heyza JR, Meek K, Schmidt JC
Nat Commun. 2024 Nov 23;15(1):10159. doi: 10.1038/s41467-024-54545-y. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
207541N-Terminal Halo XRCC4Homologous recombination donor to insert halo tag at the N terminal of the endogenous XRCC4 locus.
207546N-terminal Halo-XLFHomologous recombination donor to insert halo tag at the N terminal of the endogenous XLF locus.
207547N-terminal Halo-Ku70Homologous recombination donor to insert halo tag at the N terminal of the endogenous Ku70 locus.

Antibodies from Article