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TCAB1 prevents nucleolar accumulation of the telomerase RNA to facilitate telomerase assembly.

Klump BM, Perez GI, Patrick EM, Adams-Boone K, Cohen SB, Han L, Yu K, Schmidt JC
Cell Rep. 2023 Jun 1;42(6):112577. doi: 10.1016/j.celrep.2023.112577. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
2075783xFLAG-Halo-DyskerinCRISPR-Cas9 genome editing encoding a FLAG-HaloTag at the endogenous dyskerin locus
207579TR knockout HRDHomologous recombination donor replacing TR with a puro cassette.
207581pHAGE-UBC-MCP-mNeonGreenThe lentiviral plasmids for expression of MCP-NeonGreen
2076073xMS2 TR knockin sgRNA 1sgRNA for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus
2076083xMS2 TR knockin sgRNA 2sgRNA 2 for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus
207609sgRNA TR KO 1sgRNA 1 to knockout TR by replacement with a PuroR cassette
207610sgRNA TR KO 2sgRNA 2 to knockout TR by replacement with a PuroR cassette

Antibodies from Article