TCAB1 prevents nucleolar accumulation of the telomerase RNA to facilitate telomerase assembly.
Klump BM, Perez GI, Patrick EM, Adams-Boone K, Cohen SB, Han L, Yu K, Schmidt JC
Cell Rep. 2023 Jun 1;42(6):112577. doi: 10.1016/j.celrep.2023.112577.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
207578 | 3xFLAG-Halo-Dyskerin | CRISPR-Cas9 genome editing encoding a FLAG-HaloTag at the endogenous dyskerin locus |
207579 | TR knockout HRD | Homologous recombination donor replacing TR with a puro cassette. |
207581 | pHAGE-UBC-MCP-mNeonGreen | The lentiviral plasmids for expression of MCP-NeonGreen |
207607 | 3xMS2 TR knockin sgRNA 1 | sgRNA for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus |
207608 | 3xMS2 TR knockin sgRNA 2 | sgRNA 2 for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus |
207609 | sgRNA TR KO 1 | sgRNA 1 to knockout TR by replacement with a PuroR cassette |
207610 | sgRNA TR KO 2 | sgRNA 2 to knockout TR by replacement with a PuroR cassette |