TCAB1 prevents nucleolar accumulation of the telomerase RNA to facilitate telomerase assembly.
Klump BM, Perez GI, Patrick EM, Adams-Boone K, Cohen SB, Han L, Yu K, Schmidt JC
Cell Rep. 2023 Jun 1;42(6):112577. doi: 10.1016/j.celrep.2023.112577.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 207535 | Halo-TCAB1 HRD | Homologous recombination donor to insert HaloTag at the N-terminus of the endogenous TCAB1 locus. |
| 207577 | mCherry-Dyskerin | N-terminal mCherry-tag using SalI/EcoRI |
| 207578 | 3xFLAG-Halo-Dyskerin | CRISPR-Cas9 genome editing encoding a FLAG-HaloTag at the endogenous dyskerin locus |
| 207579 | TR knockout HRD | Homologous recombination donor replacing TR with a puro cassette. |
| 207581 | pHAGE-UBC-MCP-mNeonGreen | The lentiviral plasmids for expression of MCP-NeonGreen |
| 207582 | 3xMS-TR-100-PURO HRD | homologous recombination donor for the insertion of the 3xMS2-tag at the endogenous locus |
| 207607 | 3xMS2 TR knockin sgRNA 1 | sgRNA for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus |
| 207608 | 3xMS2 TR knockin sgRNA 2 | sgRNA 2 for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus |
| 207609 | sgRNA TR KO 1 | sgRNA 1 to knockout TR by replacement with a PuroR cassette |
| 207610 | sgRNA TR KO 2 | sgRNA 2 to knockout TR by replacement with a PuroR cassette |
| 207611 | Halo-TCAB1 KI sgRNA | sgRNA for HaloTag insertion into the endogenous TCAB1 locus |
| 207612 | pSuper TR G414C | U3 promoter driven expression of the telomerase RNA with a G414C CAB box mutation |