Genetic Encoding of 7-Aza-l-tryptophan: Isoelectronic Substitution of a Single CH-Group in a Protein for a Nitrogen Atom for Site-Selective Isotope Labeling.

Abdelkader EH, Qianzhu H, Huber T, Otting G
ACS Sens. 2023 Oct 27. doi: 10.1021/acssensors.3c01904. (Link opens in a new window) PubMed (Link opens in a new window) Article

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ID	Plasmid	Purpose
207517	pET-21(+)Tm9D8* TrpB	Expression of Tm9D8* TrpB for the synthesis of 7-Aza-L-Tryptophan (7AW) from 7-Azaindole (7AI) and serine
207516	pRSF-G1(7AW)RS	tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Aza-L-Tryptophan (7AW) into proteins in E. coli in response to the amber (TAG) codon