Modular pooled discovery of synthetic knockin sequences to program durable cell therapies.
Blaeschke F, Chen YY, Apathy R, Daniel B, Chen AY, Chen PA, Sandor K, Zhang W, Li Z, Mowery CT, Yamamoto TN, Nyberg WA, To A, Yu R, Bueno R, Kim MC, Schmidt R, Goodman DB, Feuchtinger T, Eyquem J, Jimmie Ye C, Carnevale J, Satpathy AT, Shifrut E, Roth TL, Marson A
Cell. 2023 Sep 14;186(19):4216-4234.e33. doi: 10.1016/j.cell.2023.08.013.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 207474 | plIG-106_NY-ESO-1_TCR_SR_Library | NY-ESO-1 TCR in combination with 129 surface receptors and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the TRAC locus of human T cells |
| 207475 | plIG-107_NY-ESO-1_TCR_TF_Library | NY-ESO-1 TCR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the |
| 207476 | plIG-109_CD19-BBz_CAR_SR_Library | CD19-BBz CAR in combination with 129 surface receptors and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the TRAC locus of human T cells |
| 207477 | plIG-110_CD19-BBz_CAR_TF_Library | CD19-BBz CAR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the |
| 207478 | plIG-123_HA-GD2-28z_CAR_TF_Library | HA-GD2-28z CAR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into th |
| 207479 | plIG-124_HA-GD2-28z_CAR_SR_Library | HA-GD2-28z CAR in combination with 129 surface receptors and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the TRAC locus of human T cel |
| 207480 | plIG-128_HA-GD2-28z_CAR_TFxTF_Library | HA-GD2-28z CAR in combination with ~10,000 transcription factor combinations (and related proteins) and controls (GFP/RFP combinations). Library can be used to generate HDR templates for modular poole |
| 207481 | plIG-132_CD19-28z_CAR_TF_and_SR_Library | CD19-28z CAR in combination with 129 surface receptors, 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knoc |
| 207482 | pIG-601_NY-ESO-1_TCR_GFP | This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells. |
| 207483 | pIG-602_NY-ESO-1_TCR_RFP | This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells. |
| 207484 | pIG-671_HA-GD2-28z_CAR_tNGFR | This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells. |
| 207485 | pIG-702_CD19-BBz_CAR_tNGFR | This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells. |
| 207486 | pIG-704_CD19-28z_CAR_tNGFR | This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells. |
| 207487 | pIG-727_HA-GD2-28z_CAR_RFP-tNGFR | This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells. |
| 207488 | pIG-748_HA-GD2-28z_CAR_BATF | This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells. |
| 207489 | pIG-747_CD19-28z_CAR_TFAP4 | This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells. |
| 207490 | pIG-749_HA-GD2-28z_CAR_TFAP4 | This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells. |
| 207491 | pIG-804_HA-GD2-28z_CAR_BATF-TFAP4 | This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells. |
| 207492 | pIG-805_HA-GD2-28z_CAR_BATF-RFP | This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells. |
| 207493 | pIG-806_HA-GD2-28z_CAR_RFP-TFAP4 | This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells. |
| 207494 | pIG-755_HA-GD2-28z_CAR_RFP-JUN | This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells. |
| 207495 | pIG-832_NY-ESO-1_TCR_FAS/4-1BB | This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells. |
| 207496 | pIG-833_NY-ESO-1_TCR_FAS/CD28 | This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells. |
| 207497 | pIG-834_NY-ESO-1_TCR_FAS/IL4R | This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells. |
| 207498 | pIG-835_NY-ESO-1_TCR_FAS/MyD88 | This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells. |
| 207499 | pIG-836_NY-ESO-1_TCR_FAS/OX40 | This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells. |
| 207500 | pIG-837_NY-ESO-1_TCR_tFAS | This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells. |
| 207501 | pIG-851_HA-GD2-28z_HA-TFAP4 | This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells. |
| 207502 | pIG-852_HA-GD2-28z_TFAP4-HA | This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells. |
| 207503 | pIG-858_CD19-BBz_CAR_TFAP4 | This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells. |
| 207504 | pIG-874_HA-GD2-28z_CAR_IL2RA | This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells. |
| 207505 | pIG-828_HA-GD2-28z_CAR_BATF_Retroviral | This plasmid can be used to generate retrovirus. |
| 207506 | pIG-829_HA-GD2-28z_CAR_JUN_Retroviral | This plasmid can be used to generate retrovirus. |
| 207507 | pIG-830_HA-GD2-28z_CAR_tNGFR_Retroviral | This plasmid can be used to generate retrovirus. |
| 207508 | pIG-831_HA-GD2-28z_CAR_TFAP4_Retroviral | This plasmid can be used to generate retrovirus. |