De novo DNA synthesis using polymerase-nucleotide conjugates.
Palluk S, Arlow DH, de Rond T, Barthel S, Kang JS, Bector R, Baghdassarian HM, Truong AN, Kim PW, Singh AK, Hillson NJ, Keasling JD
Nat Biotechnol. 2018 Aug;36(7):645-650. doi: 10.1038/nbt.4173. Epub 2018 Jun 18.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 207458 | pET19-MTdTc302 | Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302 |
| 207459 | pET19-M(BRCT)TdTwt | Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domain |
| 207460 | pET19-MTdTwt | Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) |
| 207461 | pET19-TdTc302 | Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302 |
| 207462 | pET19-TdTc180 | Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 180 |
| 207463 | pET19-TdTc188 | Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 188 |
| 207464 | pET19-TdTc253 | Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 253 |
| 207465 | pET19-TdTdelta5cys | Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) surface-cysteine free variant |
| 207466 | pET19-TdTwt | Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) |