Exploiting activation and inactivation mechanisms in type I-C CRISPR-Cas3 for genome-editing applications.
Hu C, Myers MT, Zhou X, Hou Z, Lozen ML, Nam KH, Zhang Y, Ke A
Mol Cell. 2024 Feb 1;84(3):463-475.e5. doi: 10.1016/j.molcel.2023.12.034. Epub 2024 Jan 18.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
207394 | pSmart-EF1alpha-NlaCas7-p65 | Expresses NlaCas7-p65 activator fusion in mammalian cells |
207395 | pSmart-EF1alpha-AcrIC8-NLS | Expresses AcrIC8 with NLS in mammalian cells |
207396 | pSmart-EF1alpha-AcrIE2-NLS | Expresses AcrIE2 with NLS in mammalian cells |
207397 | pSmart-EF1alpha-AcrIC9-NLS | Expresses AcrIC9 with NLS in mammalian cells |
207409 | pET28b-TEV-AcrIC9 | Protein purification vector to produce AcrIC9 in E. coli |
207410 | pRSFDuet-3C-AcrIC8 | Protein purification vector to produce AcrIC8 in E. coli |