Prime editing functionally corrects cystic fibrosis-causing CFTR mutations in human organoids and airway epithelial cells.
Bulcaen M, Kortleven P, Liu RB, Maule G, Dreano E, Kelly M, Ensinck MM, Thierie S, Smits M, Ciciani M, Hatton A, Chevalier B, Ramalho AS, Casadevall I Solvas X, Debyser Z, Vermeulen F, Gijsbers R, Sermet-Gaudelus I, Cereseto A, Carlon MS
Cell Rep Med. 2024 May 21;5(5):101544. doi: 10.1016/j.xcrm.2024.101544. Epub 2024 May 1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 207352 | Lenti-CMV-PE2-IRES-ZeoR | Lentiviral transfer plasmid containing CMV-driven expression cassette encoding PE2 enzyme for prime editing. |
| 207353 | Lenti-CMV-PEmax-IRES-ZeoR | Lentiviral transfer plasmid containing CMV-driven expression cassette encoding PEmax enzyme for prime editing. |
| 207354 | Lenti-CMV-MLH1dn-IRES-puroR | Lentiviral transfer plasmid containing CMV-driven expression cassette encoding MLH1dn for prime editing (PE4 and PE5 systems). |
| 207355 | Lenti-RNF2-tevopreQ1-epegRNA+5G>T_EF1a-puroR | Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 engineered prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene. |
| 207356 | Lenti-N1303K-tevopreQ1-epegRNA+13C>G_EF1a-puroR (PBS 14 - RTT 18) | Lentiviral transfer plasmid encoding hU6-driven expression of a N1303K-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene. |
| 207357 | Lenti-L227R-tevopreQ1-epegRNA+13C>A_EF1a-puroR (PBS 10 - RTT 19) | Lentiviral transfer plasmid encoding hU6-driven expression of a L227R-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene. |
| 207358 | Lenti-RNF2-ngRNA+41_EF1a-puroR | Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 nicking guide RNA and EF1a-driven puromycin resistance gene. |
| 207359 | Lenti-N1303K-ngRNA+14_EF1a-puroR | Lentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of N1303K-CFTR via prime editing and EF1a-driven puromycin resistance gene. |
| 207360 | Lenti-L227R-ngRNA+15_EF1a-puroR | Lentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of L227R-CFTR via prime editing and EF1a-driven puromycin resistance gene. |