Addgene: REGA-SIGN: Development of a Novel Set of NanoBRET-Based G Protein Biosensors.

REGA-SIGN: Development of a Novel Set of NanoBRET-Based G Protein Biosensors.

Boon K, Vanalken N, Meyen E, Schols D, Van Loy T
Biosensors (Basel). 2023 Jul 28;13(8):767. doi: 10.3390/bios13080767. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
207160	pcDNA3.1(+)Gγ2-LSS-mKATE2(N)	Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG2 tagged with LSS-mKATE2 to be paired with one of the GNA
207159	pcDNA3.1(+)Gγ1-LSS-mKATE2(N)	Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG1 tagged with LSS-mKATE2 to be paired with one of the GNA
207158	pcDNA3.1(+)GαsL-260-NLuc	Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes long transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2
207157	pcDNA3.1(+)GαsS-246-Nluc	Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes short transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2
207156	pcDNA3.1(+)Gα12-133-NLuc	Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA12 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
207155	pcDNA3.1(+)Gα13-125-NLuc	Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA13 tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2
207154	pcDNA3.1(+)Gα15-244-NLuc	Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA15 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
207153	pcDNA3.1(+)Gαq-124-NLuc	Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAQ tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
207152	pcDNA3.1(+)GαoB-91-NLuc	Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
207151	pcDNA3.1(+)GαoA-91-NLuc	Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant A of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

Antibodies from Article