The mRNA methyltransferase Mettl3 modulates cytokine mRNA stability and limits functional responses in mast cells.
Leoni C, Bataclan M, Ito-Kureha T, Heissmeyer V, Monticelli S
Nat Commun. 2023 Jun 29;14(1):3862. doi: 10.1038/s41467-023-39614-y.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 207122 | pSCALPS-FLAG-HA-mmuMettl3 | Lentiviral plasmid to express mouse Mettl3 |
| 207123 | plVX-EF1a-FLAG-hMETTL3-IRES-ZsGreen | Lentiviral vector to express human METTL3 |
| 207124 | pSCALPS-FLAG-HA-mmuMettl3 APPA | Lentiviral plasmid to express mouse Mettl3 APPA mutant |
| 207125 | pmirGLO-3'UTR mouse Il13 | Luciferase vector containing 3'UTR for mouse Il13 |
| 207126 | pmirGLO-3'UTR mouse Il6 | Luciferase vector containing 3'UTR for mouse Il6 |
| 207127 | pmirGLO-3'UTR mouse Tnf | Luciferase vector containing 3'UTR for mouse Tnf |
| 207128 | pmirGLO-3'UTR mouse Il13 5xBoxB | Tethered luciferase reporter for mouse Il13 BoxB |
| 207129 | pSCALPS-FLAG-HA-mmuMettl3-LN | Lentiviral plasmid to express mouse Mettl3 Lambda peptide C-ter |
| 207130 | pmirGLO-3'UTR mouse Il13 mutDRACH | Luciferase vector containing 3'UTR for mouse Il13 with mutated DRACH sites |
| 207132 | pSCALPS-EGFP-Cre | Lentiviral vector to express EGFP-Cre |
| 207135 | pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop cKit HDR | DNA sequence source for amplifying an HDR template for mouse cKit knockout and reporter knock-in |
| 207136 | pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop Mettl3 HDR | DNA sequence source for amplifying an HDR template for mouse Mettl3 knockout and reporter knock-in |