Membrane recruitment of Atg8 by Hfl1 facilitates turnover of vacuolar membrane proteins in yeast cells approaching stationary phase.
He CW, Cui XF, Ma SJ, Xu Q, Ran YP, Chen WZ, Mu JX, Li H, Zhu J, Gong Q, Xie Z
BMC Biol. 2021 Jun 4;19(1):117. doi: 10.1186/s12915-021-01048-7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 207006 | PCUP1-GFP-Pho8-406 | Integration of GFP-Pho8 as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Marker for yeast vacuolar membrane. |
| 207007 | PCUP1-GFP-Osh1-406 | Integration of Osh1-GFP as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Yeast PMN pathway substrate. |
| 207009 | Vph1-2GFP-URA | Integration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane. |
| 207010 | Ypq2-2GFP-URA | Integration of 2xGFP tag at YPQ2 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane. |
| 207011 | BS-TRP1-TetOff(7repeats)-GFP-Pho8 | Tet-Off controlled GFP-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid. |
| 207012 | ClhN-URA3 | Plasmid backbone for yeast expression, use auxotrophic marker URA3 (Kluyveromyces lactis) |
| 207013 | ClhN-MET15 | Plasmid backbone for yeast expression, use auxotrophic marker MET15 (Saccharomyces cerevisiae) |
| 207014 | ClhN-HIS5 | Plasmid backbone for yeast expression, use auxotrophic marker HIS5 (Schizosaccharomyces Pombe) |
| 207015 | IAA17-4flag-HIS5 | For ORF tagging with IAA17-4Flag. IAA17 is an auxin-inducible degron (AID). |
| 207016 | PADH1-TIR1-9myc-URA3 | Expresses TIR1-9Myc. TIR1 mediates auxin-induced ubiquitination of auxin-inducible degron (AID). |
| 207017 | PTPI1-Dsred-URA3 | Expresses DsRed-Express 2 as a marker of cytosol, uses auxotrophic marker URA3(Saccharomyces cerevisiae). |
| 207018 | BS-ZJLEU-PTEF1-GFP | Over-expression of GFP under TEF1 promoter, use auxotrophic marker LEU2(Saccharomyces cerevisiae). |
| 207019 | BS-ZJLEU-PTEF1-HFL1-W371A-GFP | Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae). |
| 207020 | BS-ZJLEU-PTEF1-HFL1-I375A-GFP | Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae). |
| 207021 | BS-ZJLEU-PTEF1-HFL1-D384A-GFP | Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae). |
| 207022 | BS-ZJLEU-PTEF1-HFL1-Y387A-GFP | Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae). |
| 207023 | BS-ZJLEU-PTEF1-HFL1-W371A I375A-GFP | Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae). |
| 207024 | BS-ZJLEU-PTEF1-HFL1-D384A Y387A-GFP | Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae). |
| 207025 | BS-ZJLEU-PTEF1-HFL1-W371A I375A D384A Y387A-GFP | Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae). |
| 207026 | PCUP1-GFP-Vma13-Ura | Expression of GFP-Vma13 under CPU1 promoter. |
| 207027 | PCUP1-Vac8-GFP-Ura | Expression of Vac8-GFP under CPU1 promoter. |
| 207028 | BS-Trp-PCUP1-4myc-Pho8 | Expression of 4Myc-Pho8 under CPU1 promoter. |
| 207029 | PCUP1-4V5-Pho8-Met | Expression of 4V5-Pho8 under CPU1 promoter. |
| 207030 | BS-TRP1-PGAL-GFP-Cps1 | Expression of GFP-Cps1 under GAL promoter. |
| 207031 | BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8 | Tet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid. |
| 207033 | P1K-3HA-Atg8∆GR-MET15 | Expression of 3HA-Atg8∆GR. This variant cannot be conjugated to PE. |
| 207034 | P1K-3HA-Atg8∆R-MET15 | Expression of 3HA-Atg8∆R. This variant bypasses the need of Atg4 for initial C-terminal processing. |
| 207035 | P1K-HFL1-8flag-LEU | Expression of Hfl1-8Flag. Uses auxotrophic marker LEU2(Saccharomyces cerevisiae). |
| 207043 | PCUP1-mRuby3-Pho8-406 | Expression of mRuby3-Pho8 under CPU1 promoter. |
| 207050 | ClhN-P1K-3VSV-Atg8(P85L P86L)-MET15 | Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae). |
| 207051 | ClhN-P1K-3VSV-Atg8(T56A R65A T87A)-MET15 | Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae). |
| 207052 | ClhN-P1K-3VSV-Atg8(R65A P85A P86A)-MET15 | Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae). |
| 207053 | ClhN-P1K-3VSV-Atg8(R65A P85L P86L)-MET15 | Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae). |
| 207054 | ClhN-P1K-3VSV-Atg8(L55W T56A V61W R65A)-MET15 | Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae). |
| 207055 | ClhN-P1K-3VSV-Atg8-MET15 | Expression of 3VSV-Atg8. Uses auxotrophic marker MET15(Saccharomyces cerevisiae). |