Protocol for auxin-inducible depletion of the RNA-binding protein PTBP1 in mouse embryonic stem cells.
Kainov Y, Zhuravskaya A, Makeyev EV
STAR Protoc. 2023 Oct 18;4(4):102644. doi: 10.1016/j.xpro.2023.102644.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 206995 | pML45 | Contains gRNA targeting mouse Ptbp1 gene and Cas9 ORF. |
| 206996 | pML33 | Contains OsTiR1 ORF and LoxP and Lox2272 sites. |
| 206997 | pML58 | Contains mAID-tag, mouse Ptbp1-specific homology arms and hygromycin b resistance gene. |
| 206998 | pML60 | Contains mAID-tag, mouse Ptbp1-specific homology arms and puromycin resistance gene. |
| 206999 | pML646 | Contains mAID-tag, mouse Ptbp1-specific homology arms and blasticidin S resistance gene. |