High-throughput tagging of endogenous loci for rapid characterization of protein function.
Kim J, Kratz AF, Chen S, Sheng J, Kim HK, Zhang L, Singh BK, Chavez A
Sci Adv. 2024 May 3;10(18):eadg8771. doi: 10.1126/sciadv.adg8771. Epub 2024 May 1.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 206990 | pDNR-gRNA | A plasmid for expression of SaCas9-sgRNA targeting donor plasmids |
| 206991 | pDNR-mCherry-T2A-3xPuroR-Frame 0 | Donor plasmid for Frame 0 with mCherry tag and triple Puromycin resistant marker (3xPuroR) |
| 206992 | pDNR-mCherry-T2A-3xPuroR-Frame 1 | Donor plasmid for Frame 1 with mCherry tag and triple Puromycin resistant marker (3xPuroR) |
| 206993 | pDNR-mCherry-T2A-3xPuroR-Frame 2 | Donor plasmid for Frame 2 with mCherry tag and triple Puromycin resistant marker (3xPuroR) |
| 206994 | pCAS | Plasmid expressing both SpCas9 and SaCas9 |