Genetically encoded barcodes for correlative volume electron microscopy.
Sigmund F, Berezin O, Beliakova S, Magerl B, Drawitsch M, Piovesan A, Goncalves F, Bodea SV, Winkler S, Bousraou Z, Grosshauser M, Samara E, Pujol-Marti J, Schadler S, So C, Irsen S, Walch A, Kofler F, Piraud M, Kornfeld J, Briggman K, Westmeyer GG
Nat Biotechnol. 2023 Apr 17. doi: 10.1038/s41587-023-01713-y.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 206938 | 1M-Qt-FLAG | Mammalian Expression of 1M-Qt-FLAG EMcapsulins |
| 206939 | 2M-Qt-FLAG | Mammalian Expression of 2M-Qt-FLAG EMcapsulins |
| 206940 | 3M-Qt-FLAG | Mammalian Expression of 3M-Qt-FLAG EMcapsulins |
| 206941 | 1M-Mx-FLAG | Mammalian Expression of 1M-Mx-FLAG EMcapsulins |
| 206942 | 2M-Mx-FLAG | Mammalian Expression of 2M-Mx-FLAG EMcapsulins |
| 206943 | 1M-Tm-BC2 | Mamamlian Expression of 1M-Tm-BC2Tag EMcapsulins |
| 206944 | sfGFP-SasG(2G)-mCherry | Mammalian expression of heterobifunctional SasG linkers (2G) |
| 206945 | sfGFP-SasG(3G)-mCherry | Mammalian expression of heterobifunctional SasG linkers (3G) |
| 206946 | sfGFP-SasG(4G)-mCherry | Mammalian expression of heterobifunctional SasG linkers (4G) |
| 206947 | sfGFP-SasG(5G)-mCherry | Mammalian expression of heterobifunctional SasG linkers (5G) |
| 206948 | sfGFP-SasG(6G)-mCherry | Mammalian expression of heterobifunctional SasG linkers (6G) |
| 206949 | sfGFP-SasG(7G)-mCherry | Mammalian expression of heterobifunctional SasG linkers (7G) |
| 206950 | sfGFP-SasG(8G)-mCherry | Mammalian expression of heterobifunctional SasG linkers (8G) |
| 206951 | 1M-Qt-antiGFP | Mammalian expression of 1M-Qt EMcapsulins targeting GFP |
| 206952 | 1M-Tm-antimCherry | Mammalian expression of 1M-Tm EMcapsulins targeting mCherry |
| 206953 | 1M-Qt-FLAG-TGA-rt20s-antiGFP | Mammalian expression of 1M-Qt EMcapsulins targeting GFP with stoichiometry control (~20% surface decoration) |
| 206954 | Qt-FLAG-TGA-rt9us-antiGFP | Mammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~8% surface decoration) |
| 206955 | Qt-FLAG-TAA-rt20s-antiGFP | Mammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~6% surface decoration) |
| 206956 | CaMKIIa-mScarlet-I-P2A-2M-Qt-FLAG | AAV plasmid for CaMKIIa driven co-expression of mScarlet-I and 2M-Qt-FLAG EMcapsulins |