Engineered lentivirus-derived nanoparticles (LVNPs) for delivery of CRISPR/Cas ribonucleoprotein complexes supporting base editing, prime editing and in vivo gene modification.
Haldrup J, Andersen S, Labial ARL, Wolff JH, Frandsen FP, Skov TW, Rovsing AB, Nielsen I, Jakobsen TS, Askou AL, Thomsen MK, Corydon TJ, Thomsen EA, Mikkelsen JG
Nucleic Acids Res. 2023 Sep 8:gkad676. doi: 10.1093/nar/gkad676.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 206880 | pLVNP2.0-SpCas9 | Expresses FLAG-tagged SpCas9 fused to the N-terminus of Gag/GagPol harbouring an intervening phospholipase C-δ1 pleckstrin homology (PH) domain |
| 206882 | pLVNP3.0-ABE8e | Expresses FLAG-tagged ABE8e fused to Gag through a linker sequence |
| 206883 | pLVNP3.0-PEmax | Expresses FLAG-tagged PEmax fused to Gag through a linker sequence |
| 206884 | pLVNP3.0-PEmaxRH | Expresses FLAG-tagged PEmax (lacking the RNAseH domain) fused to Gag through a linker sequence |
| 206885 | pU6-sgRNA-CBh-eGFP | cloning backbone for sgRNA using OptScf2 |