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ProDOL: a general method to determine the degree of labeling for staining optimization and molecular counting.

Tashev SA, Euchner J, Yserentant K, Hanselmann S, Hild F, Chmielewicz W, Hummert J, Schworer F, Tsopoulidis N, Germer S, Sassmannshausen Z, Fackler OT, Klingmuller U, Herten DP
Nat Methods. 2024 Sep;21(9):1708-1715. doi: 10.1038/s41592-024-02376-6. Epub 2024 Aug 8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
206866pBABE-ProDOLExpression of ProDOL probe - LynAnchor-eGFP-SNAP-tag-HaloTag
206867pBABE-LynGExpression of LynG probe - LynAnchor-eGFP

Antibodies from Article