Engineering activatable promoters for scalable and multi-input CRISPRa/i circuits.
Alba Burbano D, Cardiff RAL, Tickman BI, Kiattisewee C, Maranas CJ, Zalatan JG, Carothers JM
Proc Natl Acad Sci U S A. 2023 Jul 25;120(30):e2220358120. doi: 10.1073/pnas.2220358120. Epub 2023 Jul 18. PubMed Article
Alba Burbano D, Cardiff RAL, Tickman BI, Kiattisewee C, Maranas CJ, Zalatan JG, Carothers JM
Proc Natl Acad Sci U S A. 2023 Jul 25;120(30):e2220358120. doi: 10.1073/pnas.2220358120. Epub 2023 Jul 18. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 206791 | pCK389 | Sp.dCas9, pTet.MCP-SoxS, J23119.gRNA |
| 206792 | pDA010.188 | J23113.dCas9 |
| 206793 | pRC029 | J23107.MCP-SoxS |
| 206794 | pRC011 | J23107.MCP-SYNZIP6 |
| 206795 | pRC012 | J23107.SoxS-SYNZIP5 |
| 206796 | pRC025 | J23107.MCP-ABI |
| 206797 | pRC027 | J23107.SoxS-PYL1 |
| 206798 | pRC042 | J23107.MCP-GAI |
| 206799 | pRC043 | J23107.SoxS-GID1 |
| 206800 | pDA040.BLD7-mRFP | EL222_D7_mRFP |
| 206801 | pDA010.EL222 | J23106.EL222 |
| 206802 | pDA303.DA9.1B.mRFP | mRFP with synthetic promoter HP3 (from Figure S21) |
| 206803 | pBT009.119.DA9 | Expresses DA9 scRNA for activation of pDA303 |