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ReLo is a simple and quick colocalization assay to identify and characterize direct protein-protein interactions

Salgania HK, Metz J, Jeske M
bioRxiv 2022.03.04.482790 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
206433pAc5.1-EGFP-Aubergine 4R-K (R11K/R13K/R15K/R17K) (HK121)Aubergine expression in Schneider cells for imaging
206434pAc5.1-EGFP-Vasa 463-661 MUT (F504E) (HK196)Vasa 463-661 expression in Schneider cells for imaging
206435pAc5.1-EGFP-Vasa open (K295N) (RR228)Vasa expression in Schneider cells for imaging
206436pAc5.1-mEGFP (FspAI) (JM49)Expression in Schneider cells for imaging
206437pAc5.1-mEGFP-CAF1 isoform A (HK50)CAF1 expression in Schneider cells for imaging
206438pAc5.1-mEGFP-CAF40 isoform A (HK51)CAf40 expression in Schneider cells for imaging
206439pAc5.1-mEGFP-CCR4 isoform A (HK52)CCR4 expression in Schneider cells for imaging
206440pAc5.1-mEGFP-NOT1 isoform C (HK53)NOT1 expression in Schneider cells for imaging
206441pAc5.1-mEGFP-NOT2 isoform A (HK54)NOT1 expression in Schneider cells for imaging
206442pAc5.1-mEGFP-NOT3 isoform A (HK55)NOT3 expression in Schneider cells for imaging
206443pAc5.1-mEGFP-human FRB (HK168)FRB expression in Schneider cells for imaging
206444pAc5.1-mEGFP-human MDM2 1-118 (HK181)MDM2 1-118 expression in Schneider cells for imaging
206445pAc5.1-mCherry (FspAI) (JM65)Expression in Schneider cells for imaging
206446pAc5.1-mCherry-Bruno isoform A (JM78)Bruno expression in Schneider cells for imaging
206447pAc5.1-mCherry-Nanos isoform B (JM154)Nanos expression in Schneider cells for imaging
206448pAc5.1-λN-HA-CAF1 isoform A (HK101)CAF1 expression in Schneider cells for imaging
206449pAc5.1-λN-HA-NOT1 isoform C (JM155)Not1 expression in Schneider cells for imaging
206450pAc5.1-PH-mEGFP (FspAI) (JM50)mEGFP expression in Schneider cells for imaging
206451pAc5.1-PH-mEGFP-Cup isoform B (JM051)Cup expression in Schneider cells for imaging
206452pAc5.1-PH-mEGFP-Bruno isoform A (JM165)Bruno expression in Schneider cells for imaging
206453pAc5.1-PH-mCherry (FspAI) (HK49)Expression in Schneider cells for imaging
206454pAc5.1-PH-mCherry-CAF1 isoform A (HK96)CAF1 expression in Schneider cells for imaging
206455pAc5.1-PH-mCherry-CAF40 isoform A (HK97)CAF40 expression in Schneider cells for imaging
206456pAc5.1-PH-mCherry-NOT2 isoform A (HK99)NOT2 expression in Schneider cells for imaging
206457pAc5.1-PH-mCherry-NOT3 isoform A (HK100)NOT3 expression in Schneider cells for imaging
206458pAc5.1-PH-mCherry-human p53 1-50 (HK180)p53 1-50 expression in Schneider cells for imaging
206459pAc5.1-PH-mCherry-human FKBP12 (HK174)FKBP12 expression in Schneider cells for imaging
206460pAc5.1-PH-mCherry-Oskar 139-606 isoform A (Short Oskar) (HK195)Oskar 139-606 expression in Schneider cells for imaging
206461pAc5.1-PH-mCherry-Oskar 139-240 (eLOTUS domain) (HK73)Oskar 139-240 expression in Schneider cells for imaging
206462pAc5.1-PH-mCherry-Oskar 241-387 (DR) (HK74)Oskar 240-387 expression in Schneider cells for imaging
206463pAc5.1-PH-mCherry-Oskar 388-606 (OSK domain) (HK75)Oskar 388-606 expression in Schneider cells for imaging
206464pAc5.1-PH-mCherry-Oskar 139-240 MUT (A162E/L228E) (HK202)Oskar 139-240 mut expression in Schneider cells for imaging
206465pAc5.1-PH-mCherry-Tudor isoform A (HK130)Tudor expression in Schneider cells for imaging
206466pAc5.1-PH-mCherry-Bam isoform A (HK62)Bam expression in Schneider cells for imaging
206467pAc5.1-PH-mCherry-Bam M24E (HK88)Bam expression in Schneider cells for imaging
206468pAc5.1-PH-mCherry-Nanos (HK65)Nanos expression in Schneider cells for imaging
206469pAc5.1-PH-mCherry-Roquin isoform A (HK63)Roquin expression in Schneider cells for imaging
206470pAc5.1-PH-mCherry-MARF1 isoform D (HK56)MARF1 expression in Schneider cells for imaging
206471pAc5.1-PH-mCherry-Bruno isoform A (HK72)Bruno expression in Schneider cells for imaging
206472pAc5.1-(FspAI)-mCherry-PH (EB3)Expression in Schneider cells for imaging
206473pAc5.1-CCR4 isoform A-mCherry-PH (EB7)CCR4 expression in Schneider cells for imaging
206474pAc5.1-NOT1 isoform C-mCherry-PH (EB5)NOT1 expression in Schneider cells for imaging
206475pAc5.1-OST4-mCherry (EcoRV) (XH26)Expression in Schneider cells for imaging
206476pAc5.1-OST4-mCherry-CAF1 isoform A (HK184)CAF1 expression in Schneider cells for imaging
206481T5-pAc5.1 EGFP-EcoRVSchneider cell imaging
206482F20-pAc5.1 EGFP AubergineAubergine expression for Schneider cell imaging
206483F15-pAc5.1 EGFP VasaVasa expression for Schneider cell imaging
206484F17-pAc5.1 EGFP Vasa 1-200vasa 1-200 expression for Schneider cell imaging
206485F18-pAc5.1 EGFP Vasa 200-462Vasa 200-642 expression for Schneider cell imaging
206486F19-pAc5.1 EGFP Vasa 463-661Vasa 463-661 expression for Schneider cell imaging
206487F16-pAc5.1 EGFP Vasa closedVasa (closed conformation) expression for Schneider cell imaging
206488F23-pAc5.1 EGFP CupCup expression for Schneider cell imaging
206489T6-pAC5.1 mEGFP-EcoRVSchneider cell imaging
206490T7-pAc5.1 mCherry EcoRVSchneider cell imaging
206491H2-pAc5.1 mCherry Oskar 139-606Oskar 139-606 expression for Schneider cell imaging
206492T8-pAc5.1 lambdaN HA EcoRVSchneider cell imaging
206493I14-pAc5.1 lambda N HA CupCup expression forSchneider cell imaging
206494H3-pAc5.1 OST4 mCherry Oskar 139-606Oskar 139-606 expression for Schneider cell imaging

Antibodies from Article