Identification of SaCas9 orthologs containing a conserved serine residue that determines simple NNGG PAM recognition.
Wang S, Tao C, Mao H, Hou L, Wang Y, Qi T, Yang Y, Ong SG, Hu S, Chai R, Wang Y
PLoS Biol. 2022 Nov 30;20(11):e3001897. doi: 10.1371/journal.pbio.3001897. eCollection 2022 Nov.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 192139 | SpeCas9 | Expresses SpeCas9, and cloning backbone for sgRNA |
| 192140 | Sha2Cas9 | Expresses Sha2Cas9, and cloning backbone for sgRNA |
| 192141 | SpeCas9-HF | Expresses highly specific SpeCas9, and cloning backbone for sgRNA |
| 192142 | Sha2Cas9-HF | Expresses highly specific Sha2Cas9, and cloning backbone for sgRNA |