Genome editing with natural and engineered CjCas9 orthologs.
Gao S, Wang Y, Qi T, Wei J, Hu Z, Liu J, Sun S, Liu H, Wang Y
Mol Ther. 2023 Apr 5;31(4):1177-1187. doi: 10.1016/j.ymthe.2023.01.029. Epub 2023 Feb 1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 192125 | pAAV-CMV-Hsp1Cas9 | Expresses Hsp1Cas9 |
| 192126 | pAAV-CMV-Hsp2Cas9 | Expresses Hsp2Cas9 |
| 192127 | pAAV-CMV-CcuCas9 | Expresses CcuCas9 |
| 192128 | pSK-mU6-Cj-sgRNA-SV40-puro | Expresses Cj-SgRNA scaffold cloned with BbsI and puromycin resistance gene |
| 192129 | pSK-mU6-Hsp1-sgRNA-SV40-puro | Expresses Hsp1-SgRNA scaffold cloned with BbsI and puromycin resistance gene |
| 192130 | pSK-mU6-Ccu-sgRNA-SV40-puro | Expresses Ccu-SgRNA scaffold cloned with BbsI and puromycin resistance gene |
| 192131 | pAAV-CMV-Hsp1-Hsp2Cas9-P2A-puro | Expresses Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene |
| 192132 | pAAV-CMV-Hsp1-Hsp2Cas9-Y-P2A-puro | Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene |
| 192133 | pAAV-CMV-Hsp1-Hsp2Cas9-KY-P2A-puro | Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene |