Non-cell-Autonomous Cardiomyocyte Regulation Complicates Gene Supplementation Therapy for Lmna-Associated Cardiac Defects in Mice.
Sun Y, Guo C, Chen Z, Lin J, Yang L, Zhang Y, Wu C, Zhao D, Jardin B, Pu WT, Zhao M, Dong E, Hu X, Zhang S, Guo Y
J Am Coll Cardiol Basic Trans Science. null2024, 0 (0).
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 206178 | pAAV-Tnnt2-Cre-U6-Lmna-sgRNA | Expresses Cre recombinase specifically in cardiomyocytes and uses U6 promoter to express sgRNAs targeting murine Lmna exon 10 |
| 206197 | pAAV-CMV-GFP-LA | Expresses GFP-tagged human Lamin-A protein by the constitutive CMV promoter in mice |
| 206198 | pAAV-Tnnt2-GFP-LA | Expresses GFP-tagged Lamin-A specifically in cardiomyocytes by the Tnnt2 promoter |
| 206199 | pAAV-Loxp-CMV-Loxp-GFP-LA | Expresses GFP-lamin-A only in non-myocyte when delivered into mice carrying a cardiomyocyte-specific Myh6-Cre transgene |