Annette Dolphin Lab: Dolphin Lab Plasmids 2023

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
206055	GFP Cav2.2 HA L702 P pCAGGS	expression of rabbit Cav2.2 with N-terminal GFP tag and exofacial double HA tag in domain II and a L702P mutation that allows trafficking to the plasma membrane but channel is not functional
206056	GFP Cav2.2 HA L702P pcDNA3	expression of rabbit Cav2.2 with a N-terminal GFP tag and an exofacial double HA tag in domain II and a L702P mutation that allows trafficking to the plasma membrane but channel is not functional
206073	Cav2.2 pHfluorin pCAGGS	expression of rabbit Cav2.2 calcium channel with an exofacial pHfluorin tag in domain II
206090	mEos Cav2.2 W391A pRK5	expression of rabbit Cav2.2 calcium channel with a N-terminal mEos tag and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2
206091	mEos Cav2.2 pRK5	expression of rabbit Cav2.2 calcium channel with a N-terminal mEos tag
206092	mEos Cav2.2 pMT2	expression of rabbit Cav2.2 calcium channel with a N-terminal mEos tag
206102	cacnb1b pCAGGS	expression of rat beta1b calcium channel auxillary subunit
206103	cacna2d1 pcDNA3	expression of rat alpha2delta-1 calcium channel auxillary subunit
206104	cacna2d1 pCAGGS	expression of rat alpha2delta-1 calcium channel auxillary subunit
206105	cacnb1b pcDNA3	expression of rat beta1b calcium channel auxillary subunit
206109	cacna2d1 R241A D491A HA pcDNA3	expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with R421A and D491A mutations that both disrupt Gabapentin binding
206113	GFP Cav2.2 HA D665A pcDNA3	expression of rabbit Cav2.2 with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D665A mutation that allows trafficking to the plasma membrane but channel is not functional
206117	cacna2d3 BBS pMT2	expression of mouse calcium channel alpha2delta-3 with a single Bungarotoxin binding site motif inserted after aa595