Functional exofacially tagged N-type calcium channels elucidate the interaction with auxiliary alpha2delta-1 subunits.
Cassidy JS, Ferron L, Kadurin I, Pratt WS, Dolphin AC
Proc Natl Acad Sci U S A. 2014 Jun 17;111(24):8979-84. doi: 10.1073/pnas.1403731111. Epub 2014 Jun 2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 206051 | Cav2.2 HA pcDNA3 | expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II |
| 206052 | Cav2.2 HA pCAGGS | expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II |
| 206057 | Cav2.2 BBS pcDNA3 | expression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II |
| 206077 | Cav2.2 BBS W391A pcDNA3 | Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop that prevents Beta binding |
| 206110 | cacna2d1 R241A pMT2 | expression of rat alpha2delta-1 calcium channel auxillary subunit and with R421A mutation that disrupts Gabapentin binding |
| 206111 | cacna2d1 R241A HA pMT2 | expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with an R421A mutation that disrupts Gabapentin binding |