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Endogenous tagging using split mNeonGreen in human iPSCs for live imaging studies.

Husser MC, Pham NP, Law C, Araujo FRB, Martin VJJ, Piekny A
Elife. 2024 Apr 23;12:RP92819. doi: 10.7554/eLife.92819. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
206042pAAVS1-Puro-CAG-mNG2(1-10)Repair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.
206043pH2B-mNG2(11)Plasmid expressing a H2B-mNG2(11) fusion in mammalian cells for complementation with the mNG2(1-10) fragment.

Antibodies from Article